The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 25, 2014
Filed:
Jun. 02, 2004
Christa Schleper, Klosterneuburg, AT;
Melanie Jonuscheit, Darmstadt, DE;
Jürgen Eck, Bensheim, DE;
Frank Niehaus, Heppenheim, DE;
Sonja-verena Albers, ST Groningen, DE;
Sabrina Froels, Dreieich, DE;
Christa Schleper, Klosterneuburg, AT;
Melanie Jonuscheit, Darmstadt, DE;
Jürgen Eck, Bensheim, DE;
Frank Niehaus, Heppenheim, DE;
Sonja-Verena Albers, ST Groningen, DE;
Sabrina Froels, Dreieich, DE;
Abstract
The present invention relates to a sulfolobus expression vector comprising: (a) sulfolobus origin of replication; (b) the genes encoding the structural proteins and the site-specific integrase of SSV1, SSV2 or pSSVx, operatively linked to expression control sequences and a packaging signal; (c) one or more selectable marker gene(s), operatively linked to sulfolobus expression control sequences; and (d) a sulfolobus promoter followed 3' by a restriction enzyme recognition site or a multiple cloning site for insertion of a gene of interest and optionally a 3′ regulatory element. Moreover, the present invention relates to a shuttle vector comprising the sequences of the expression vector of the invention and additional sequences for propagation and selection in, wherein the additional sequences comprise (a) anon of replication; and (b) a marker for selection in. Furthermore, the invention relates to host cells transformed with the expression vector as well as to a kit comprising a vector or a host cell of the present invention. Finally, the present application also relates to a method for generating infectious subviral particles.