The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 25, 2014

Filed:

Jul. 21, 2008
Applicants:

Kalle Levon, Brooklyn, NY (US);

Bin Yu, West Hartford, CT (US);

Yanxiu Zhou, West Hartford, CT (US);

Inventors:

Kalle Levon, Brooklyn, NY (US);

Bin Yu, West Hartford, CT (US);

Yanxiu Zhou, West Hartford, CT (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 13/00 (2006.01); C12N 11/00 (2006.01); G01N 33/53 (2006.01); C12Q 1/00 (2006.01);
U.S. Cl.
CPC ...
Abstract

A real-time method employing a portable peptide-containing potentiometric biosensor, can directly detect and/or quantify bacterial spores. Two peptides for specific recognition ofandSterne may be immobilized by a polysiloxane monolayer immobilization (PMI) technique. The sensors translate the biological recognition event into a potential change by detecting, for example,spores in a concentration range of 0.08-7.3×10CFU/ml. The sensing method exhibited highly selective recognition properties towardsspores over other kinds of spores. The selectivity coefficients of the sensors for other kinds of spores are in the range of 0-1.0×10. The biosensor method not only has the specificity to distinguishspores in a mixture ofand(.) Kurstaki spores, but also can discriminate between live and deadspores. Furthermore, the sensing method can distinguish aAfrom otherstrain. Assay time may be as low as about 5 minutes for a single test. Rapid identification ofSterne andΔAmes was also provided.


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