The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 25, 2014
Filed:
Mar. 03, 2009
Hrair Kirakossian, San Jose, CA (US);
Hrair Kirakossian, San Jose, CA (US);
Becton, Dickinson and Company, Franklin Lakes, NJ (US);
Abstract
The invention provides a low-cost method for identifying and/or measuring a subpopulation of cells in a biological sample, such as a subpopulation of CD4-positive cells in a whole blood sample. In accordance with the invention, one or more cell surface markers are identified on a subpopulation of interest, which collectively are present in higher numbers on the cells of the subpopulation than on cells not in the subpopulation of interest. A probe comprising a binding compound and a density label are combined with the sample and the resulting mixture is centrifuged or sedimented through a density medium in a capillary. Labeled cells of interest pass through the density medium and stack at the bottom of the capillary where they may be visually detected and quantified by the height of the stack.