The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 25, 2014
Filed:
Jul. 20, 2011
Ann Dak-yee Kwong, Cambridge, MA (US);
James Daniel Frantz, Belmont, MA (US);
Douglas John Bartels, North Liberty, IA (US);
Chao Lin, Winchester, MA (US);
Benjamin Shames, Arlington, MA (US);
Sheila Seepersaud, Acton, MA (US);
Judith A. Lippke, Chestnut Hill, MA (US);
Tara Lynn Kieffer, Brookline, MA (US);
Yi Zhou, Lexington, MA (US);
Eileen Z. Zhang, Andover, MA (US);
James C. Sullivan, Sudbury, MA (US);
Ann Dak-Yee Kwong, Cambridge, MA (US);
James Daniel Frantz, Belmont, MA (US);
Douglas John Bartels, North Liberty, IA (US);
Chao Lin, Winchester, MA (US);
Benjamin Shames, Arlington, MA (US);
Sheila Seepersaud, Acton, MA (US);
Judith A. Lippke, Chestnut Hill, MA (US);
Tara Lynn Kieffer, Brookline, MA (US);
Yi Zhou, Lexington, MA (US);
Eileen Z. Zhang, Andover, MA (US);
James C. Sullivan, Sudbury, MA (US);
Vertex Pharmaceuticals Incorporated, Boston, MA (US);
Abstract
A method of amplifying an HCV nucleic acid in an HCV infected sample comprises amplifying a segment of a DNA template that is complementary to a genome of HCV RNA from the sample by a two-stage PCR, wherein a first stage PCR employs a first outer primer and a second outer primer, and a second stage PCR employs a first inner primer and a second inner primer. The nucleotide sequence of the first outer primer comprises a nucleotide sequence as set forth in SEQ ID NO: 2; or SEQ ID NO:9, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 9. The nucleotide sequence of the second outer primer comprises a nucleotide sequence set forth in SEQ ID NO: 3 or 4; or a nucleotide sequence as set forth in SEQ ID NO: 10 or 11, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 10 and 11. The nucleotide sequence of the first inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 5; or SEQ ID NO:12, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 12. The nucleotide sequence of the second inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 6 or 7; or a nucleotide sequence as set forth in SEQ ID NO: 13 or 14, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 13 and 14.