The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 11, 2014
Filed:
Dec. 12, 2011
Haohui Kong, Guangdong, CN;
Yifei Huang, Guangdong, CN;
Baofeng Jin, Guangdong, CN;
China Tobacco Guangdong Industrial Co., Ltd., Guangdong, CN;
Abstract
A method for determining pectin content in a plant sample includes: 1) adding an acidic alcohol solution to the plant sample, heating in a water bath and filtration; 2) soaking residue from step 1 with an acidic solution, heating in a water bath, a second filtration, bringing to volume after cooling and obtaining filtrate; 3) adding an acetic acid/sodium acetate buffer solution to residue of step 2, adding a pectinase solution, heating under vibration in a water bath, and a third filtration to obtain a filtrate; 4) adding an acetic acid/sodium acetate buffer solution and a pectinase solution to filtrate from step 2, heating the mixture under vibration in a water bath to obtain an enzymatic hydrolysate, adding filtrate obtained in step 3 to the enzymatic hydrolysate, bringing to volume, and obtaining a test solution; 5) drawing the test solution into a continuous flow analyzer to perform analysis.