The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 11, 2014
Filed:
Jun. 16, 2009
Applicants:
Amar Gupta, Danville, CA (US);
Stephen Gordon Will, Oakland, CA (US);
Roy Bohenzky, Mountain View, CA (US);
Janine Montiel, Fremont, CA (US);
Inventors:
Amar Gupta, Danville, CA (US);
Stephen Gordon Will, Oakland, CA (US);
Roy Bohenzky, Mountain View, CA (US);
Janine Montiel, Fremont, CA (US);
Assignee:
Roche Molecular Systems, Inc., Pleasanton, CA (US);
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01);
U.S. Cl.
CPC ...
Abstract
An improved method of preventing carryover contamination of an amplification reaction involves treating uracil-containing DNA with uracil-N-DNA glycosylase and heating the DNA in the presence of polyamines, such as spermidine, spermine and the like. Alternatively, after treatment with uracil-N-DNA glycosylase, the reaction is further incubated with an enzyme having AP lyase activity.