The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 04, 2014
Filed:
Mar. 16, 2009
Hiroaki Kataoka, Miyazaki, JP;
Hirotake Takai, Shizuoka, JP;
Atsuhiko Kato, Shizuoka, JP;
Masami Suzuki, Shizuoka, JP;
Masamichi Sugimoto, Kanagawa, JP;
Hiroaki Kataoka, Miyazaki, JP;
Hirotake Takai, Shizuoka, JP;
Atsuhiko Kato, Shizuoka, JP;
Masami Suzuki, Shizuoka, JP;
Masamichi Sugimoto, Kanagawa, JP;
University of Miyazaki, Miyazaki, JP;
Chugai Seiyaku Kabushiki Kaisha, Tokyo, JP;
Abstract
The present invention relates to an in-vitro immunoassay method for detecting the presence of liver cancer cells in a subject. In the method of the present invention, antigen retrieval treatment based on heat-induced epitope retrieval method and antigen retrieval treatment based on protease-induced epitope retrieval method can be combined in the detection of glypican 3 antigen expression in liver cancer tissues to thereby detect the difference in the expression level or expression pattern of the glypican 3 antigen by immunohistochemical staining method. This enables samples, which has been determined by the conventional HIER method as highly expressing glypican 3, to be graded according to the expression level of glypican 3.