The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 25, 2014
Filed:
Mar. 22, 2012
Thang Pham, Mountain View, CA (US);
Arunashree Bhamidipati, Palo Alto, CA (US);
Kevin Travers, Menlo Park, CA (US);
Eric Olivares, Union City, CA (US);
Tyson A. Clark, Menlo Park, CA (US);
Jonas Korlach, Newark, CA (US);
Thang Pham, Mountain View, CA (US);
Arunashree Bhamidipati, Palo Alto, CA (US);
Kevin Travers, Menlo Park, CA (US);
Eric Olivares, Union City, CA (US);
Tyson A. Clark, Menlo Park, CA (US);
Jonas Korlach, Newark, CA (US);
Pacific Biosciences of California, Inc., Menlo Park, CA (US);
Abstract
Compositions, methods and systems are provided for the isolation of polymerase-nucleic acid complexes. Complexes can be separated from free enzyme by using hook molecules to target single stranded regions on the nucleic acid. Active complexes can be isolated from mixtures having both active and inactive complexes by initiating nucleic acid synthesis so as to open up a portion of a double stranded region rendering that region single stranded. Hook molecules are targeted to bind the sequences that are thus exposed. The hook molecules bound to active polymerase-nucleic acid complex are isolated, and the active polymerase-nucleic acid complexes released.