The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 18, 2014

Filed:

Jul. 06, 2009
Applicants:

Kathleen D′halluin, Mariakerke, BE;

Chantal Vanderstraeten, Ghent, BE;

Rene Ruiter, Huesden, BE;

Inventors:

Kathleen D′Halluin, Mariakerke, BE;

Chantal Vanderstraeten, Ghent, BE;

Rene Ruiter, Huesden, BE;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/82 (2006.01);
U.S. Cl.
CPC ...
Abstract

Methods and means are provided to improve targeted DNA insertion in plants using rare-cleaving 'double stranded break' inducing enzymes. More specifically, the methods involve introducing the DNA that is to be inserted into the induced break into the plant cell via direct DNA delivery and incubating the plant cells in a plant phenolic compound prior to DNA delivery. Also provided are improved I-SceI encoding nucleotide sequences having a CG content of 50-60%.


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