The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 11, 2014
Filed:
Sep. 30, 2009
Jeffrey Shasky, Davis, CA (US);
Wendy Yoder, Davis, CA (US);
Jeffrey Shasky, Davis, CA (US);
Wendy Yoder, Davis, CA (US);
Novozymes Inc., Davis, CA (US);
Abstract
The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parentstrain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5'-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parentstrain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants ofstrains and methods for producing such mutants.