The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 04, 2014
Filed:
Sep. 13, 2011
Applicants:
Wei-kuang Liu, Taipei, TW;
Min-pey Ding, Taipei County, TW;
Inventors:
Wei-Kuang Liu, Taipei, TW;
Min-Pey Ding, Taipei County, TW;
Assignee:
Scinopharm Taiwan, Ltd., Tainan County, TW;
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/00 (2006.01);
U.S. Cl.
CPC ...
Abstract
A method for screening cells with high level expression of a target protein is disclosed. The method includes introducing into a plurality of host cells a DNA construct that encodes both a target protein and an inhibitor to an endogenous selectable marker in the host cells, screening host cells harboring the DNA construct for the expression of the endogenous selectable marker, and isolating cells with reduced expression of the selectable marker. Also disclosed is a DNA construct configured to express both the target protein and the inhibitor inside the host cell.