The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 07, 2014
Filed:
Jun. 30, 2011
Yan Sun, Tianjin, CN;
Guozhen Wang, Tianjin, CN;
Qinghong Shi, Tianjin, CN;
Xiaoyan Dong, Tianjin, CN;
Yan Sun, Tianjin, CN;
Guozhen Wang, Tianjin, CN;
Qinghong Shi, Tianjin, CN;
Xiaoyan Dong, Tianjin, CN;
Tianjin University, Tianjin, CN;
Abstract
A method for protein refolding with an ion exchange resin. The method includes (a) choosing an ion exchange resin that having charged groups with the same sign as a net charge of a denatured protein to be refolded; (b) removing heterogeneous ions from the ion exchange resin by washing the ion exchange resin sequentially with saline solution and deionized water, to prepare the ion exchange resin; (c) mixing the ion exchange resin with a refolding buffer thoroughly, then adding the denatured protein to the refolding buffer and allowing the denatured protein to refold; and then (d) collecting the supernatant by centrifugation or settlement, to obtain a solution containing the refolded protein.