The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 07, 2014
Filed:
Apr. 06, 2010
Eric Steinmetz, Madison, WI (US);
Ronald Godiska, Mount Horeb, WI (US);
David A. Mead, Middleton, WI (US);
Eric Steinmetz, Madison, WI (US);
Ronald Godiska, Mount Horeb, WI (US);
David A. Mead, Middleton, WI (US);
Lucigen Corporation, Middleton, WI (US);
Abstract
A system for ligase-free cloning and/or expressing a target gene is described herein. A preferred version of the invention includes anhost. The host preferably includes a T7 RNA polymerase gene comprising a T7gpl coding sequence, a lacUV5 promoter, and a lac operator. The host preferably further includes a lacI gene comprising a lacI coding sequence with an ATG start codon, a promoter derived from the lacallele, and a translational enhancer derived from a 5' RNA leader sequence of T7 gene 10. The invention further includes a low-copy plasmid vector comprising a T7 promoter a lac operator operationally linked to the T7 promoter. The system is configured to inhibit target gene expression when uninduced and to permit gene expression upon induction by auto-induction.