The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 10, 2013
Filed:
Jul. 25, 2011
Michel Chartrain, Westfield, NJ (US);
Laura Kizer Bentley, Monmouth, NJ (US);
Barbara Ann Krulewicz, Bloomfield, NJ (US);
Kristin M. Listner, East Windsor, NJ (US);
Wen-jun Sun, Edison, NJ (US);
Chanyong Brian Lee, Thousand Oaks, CA (US);
Michel Chartrain, Westfield, NJ (US);
Laura Kizer Bentley, Monmouth, NJ (US);
Barbara Ann Krulewicz, Bloomfield, NJ (US);
Kristin M. Listner, East Windsor, NJ (US);
Wen-jun Sun, Edison, NJ (US);
Chanyong Brian Lee, Thousand Oaks, CA (US);
Merck Sharp & Dohme Corp., Rahway, NJ (US);
Abstract
The present invention relates generally to a method for increasing the yield of plasmid DNA production. The method includes the steps of selecting a highly productive clonal subtype of a strain of, including but not limited to the DH5 strain, harboring a DNA plasmid and cultivating said clonal subtype with fed-batch fermentation in a chemically-defined medium. The plasmid DNA production process described herein can generate record quantities of plasmid DNA when said highly productive clonal subtypes are cultivated on an industrial scale. The disclosed method can be used for the production of pharmaceutical grade DNA for use in polynucleotide vaccination and gene therapy treatment regimens.