The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 10, 2013
Filed:
Sep. 25, 1991
Frederick S. Hagen, King County, WA (US);
Mark J. Murray, King County, WA (US);
Sharon J. Busby, King County, WA (US);
Kathleen L. Berkner, King County, WA (US);
Margaret Y. Insley, King County, WA (US);
Richard G. Woodbury, King County, WA (US);
Charles L. Gray, King County, WA (US);
Frederick S. Hagen, King County, WA (US);
Mark J. Murray, King County, WA (US);
Sharon J. Busby, King County, WA (US);
Kathleen L. Berkner, King County, WA (US);
Margaret Y. Insley, King County, WA (US);
Richard G. Woodbury, King County, WA (US);
Charles L. Gray, King County, WA (US);
ZymoGenetrics, Inc., Seattle, WA (US);
Abstract
Methods are disclosed for producing proteins having biological activity for blood coagulation mediated by Factor VIIa or Factor IX. The proteins are produced by mammalian host cells which have been stably transfected with a DNA construct containing a nucleotide sequence which codes at least partially for either Factor VII or Factor IX. The nucleotide sequence comprises a first nucleotide sequence encoding a calcium binding domain, joined to a second nucleotide sequence positioned downstream of the first sequence. The second sequence encodes a catalytic domain for the serine protease activity of either Factor VIIa or Factor IX. The joined sequences code for proteins having substantially the same biological activity for blood coagulation as either Factor VIIa or Factor IX.