The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 26, 2013
Filed:
Mar. 05, 2010
Jin Ryoun Kim, Jericho, NY (US);
Brennal Pierre, Brooklyn, NY (US);
Marc Ostermeier, Baltimore, MD (US);
Chung-sei Kim, Palisade Park, NJ (US);
Jin Ryoun Kim, Jericho, NY (US);
Brennal Pierre, Brooklyn, NY (US);
Marc Ostermeier, Baltimore, MD (US);
Chung-Sei Kim, Palisade Park, NJ (US);
Polytechnic Institute of NYU, Brooklyn, NY (US);
Abstract
A strategy to improve protein stability by domain insertion. TEM 1 beta-lactamase (BLA) and exo-inulinase, as model target enzymes, are inserted into a hyperthermophilic maltose binding protein from(PfMBP). Unlike conventional protein stabilization methods that employ mutations and recombinations, the inventive approach does not require any modification on a target protein except for its connection with a hyperthermophilic protein scaffold. For that reason, target protein substrate specificity was largely maintained, which is often modified through conventional protein stabilization methods. The insertion was achieved through gene fusion by recombinant DNA techniques.