The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 19, 2013

Filed:

Aug. 29, 2008
Applicants:

Shinji Yamasaki, Sakai, JP;

Worada Samosorunsuk, Sakai, JP;

Masahiro Asakura, Osaka, JP;

Inventors:

Shinji Yamasaki, Sakai, JP;

Worada Samosorunsuk, Sakai, JP;

Masahiro Asakura, Osaka, JP;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/06 (2006.01); C12N 15/00 (2006.01); C07H 21/02 (2006.01); C07K 14/00 (2006.01);
U.S. Cl.
CPC ...
Abstract

An objective of the present invention is to provide the cytolethal distending toxin (CDT) ofand polynucleotides encoding it, and novel methods for detection ofusing the cdt genes. The present inventors focused on the cytolethal distending toxin (CDT) ofbacteria, and detected the cdt genes of a-like bacterium isolated from an enteritis patient in Thailand. The present inventors discovered a bacterial strain whose cdtB gene was amplified by common primers in, and, but not by multiplex PCR that can specifically detect the cdtA, cdtB, and cdtC genes of the three bacterial species. The bacterial strain was identified asby 16S rRNA gene analysis. Furthermore, the entire nucleotide sequence of the cdt genes was determined by genome walking upstream and downstream of the cdtB gene.


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