The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 05, 2013

Filed:

Nov. 03, 2010
Applicants:

Ramesh Vaidyanathan, Madison, WI (US);

Scott Kuersten, Fitchburg, WI (US);

Ken Doyle, Fitchburg, WI (US);

Inventors:

Ramesh Vaidyanathan, Madison, WI (US);

Scott Kuersten, Fitchburg, WI (US);

Ken Doyle, Fitchburg, WI (US);

Assignee:
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/42 (2006.01);
U.S. Cl.
CPC ...
Abstract

The present innovation provides methods and kits that enable rapid and efficient dual end-tagging of RNA to prepare libraries for analysis by applications such as next-generation RNA sequencing, qPCR, microarray analysis, or cloning. The methods do not require time-consuming and inefficient gel-purification steps that are common to methods known in the art. In addition, the present invention provides methods and kits for rapid, high-throughput enzymatic preparation of 5'-activated, 3′-blocked DNA oligonucleotides from standard, single-stranded DNA oligonucleotides.


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