The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 05, 2013
Filed:
Feb. 12, 2007
Pina Fratamico, Elkins Park, PA (US);
Susumu Kawasaki, Tsukuba, JP;
Shinichi Kawamoto, Tsukuba, JP;
The United States of America, as represented by the Secretary of Agriculture, Washington, DC (US);
National Agriculture & Food Research Organization, Tsukuba Ibaraki, JP;
Abstract
The phylogeny of twelvespecies was determined based on partial (1020-bp) gyrB gene sequences. Methods have been described for detection and speciation of, including 16S rRNA sequence analysis. However, gyrB provides a better resolution than the 16S rDNA gene forspecies with interspecies sequence similarities ranging from 58.3 to 89.2% compared to those reported for the 16S rRNA gene (ranging from 89 to 99%). A universal primer set, designed to amplify a 900-bp fragment of the gyrB gene inspp., was developed and used for PCR-RFLP of 19 strains representing twelvespecies and resulted in unique digest patterns for all twelvespecies PCR assays for amplification of regions of the gyrB gene specific for eachspecies were also developed. Using these PCR and PCR-RFLP methods results in unambiguous identification of the majority ofspecies.