The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 15, 2013
Filed:
Apr. 06, 2012
Johann Engelhardt, Bad Schoenborn, DE;
Stefan W. Hell, Goettingen, DE;
Jan Keller-findeisen, Geottingen, DE;
Johann Engelhardt, Bad Schoenborn, DE;
Stefan W. Hell, Goettingen, DE;
Jan Keller-Findeisen, Geottingen, DE;
Deutsches Krebsforschungszentrum, Heidelberg, DE;
Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V., Munich, DE;
Abstract
To the end of three-dimensionally localizing light emitting marker entities of unknown orientation and unknown position in a sample, the light emitted by each single marker entity is imaged in at least two different ways onto at least one detection plane which corresponds to a focal plane () in the sample resulting in at least two images of the marker entity. Virtual x- and y-positions of the marker entity in parallel to the focal plane () are separately determined from the emitted light intensity distribution over each image of the marker entity. Further, the z-position of the marker entity normal to the focal plane is determined from the emitted light intensity distributions over the images of the marker entity. The real x- and y-positions of the marker entity in parallel to the focal plane () are determined based on its virtual x- and y-positions and on its z-position.