The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 15, 2013
Filed:
Mar. 11, 2008
Masanao Watanabe, Tokyo, JP;
Kenichi Hagiwara, Tokyo, JP;
Teruo Okano, Tokyo, JP;
Masayuki Yamato, Tokyo, JP;
Tatsuya Shimizu, Tokyo, JP;
Yoshikatsu Akiyama, Tokyo, JP;
Hiroya Watanabe, Tokyo, JP;
Keisuke Ashiba, Tokyo, JP;
Masanao Watanabe, Tokyo, JP;
Kenichi Hagiwara, Tokyo, JP;
Teruo Okano, Tokyo, JP;
Masayuki Yamato, Tokyo, JP;
Tatsuya Shimizu, Tokyo, JP;
Yoshikatsu Akiyama, Tokyo, JP;
Hiroya Watanabe, Tokyo, JP;
Keisuke Ashiba, Tokyo, JP;
Dai Nippon Printing Co., Ltd., Tokyo, JP;
Tokyo Woman's Medical University, Tokyo, JP;
Cellseed Inc., Tokyo, JP;
Abstract
An object of the present invention is to provide a cell culture support making the detachment of a cell sheet easy as well as enabling the formation of a uniform cell sheet. The present invention relates to a method for manufacturing a cell culture support having a temperature responsive polymer immobilized onto the surface thereof via covalent bonding, the method including a coating step in which a composition including a monomer that can form the polymer by polymerization by radiation irradiation, an organic solvent and, in some cases, a prepolymer formed by polymerization of the monomer is coated onto the substrate having a surface containing a material which can be covalently bonded to the temperature responsive polymer by radiation irradiation to form a film on the surface of the substrate, a radiation irradiation step in which a polymerization reaction and a binding reaction between the substrate surface and the temperature responsive polymer are allowed to proceed by irradiating radiation to the film, and a drying step to dry the film.