The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 01, 2013
Filed:
Sep. 10, 2010
Wei HU, Beijing, CN;
Zuoyan Zhu, Beijing, CN;
Hong MA, Beijing, CN;
BO Guan, Beijing, CN;
Yuanlei HU, Beijing, CN;
Zhongping Lin, Beijing, CN;
Wei Hu, Beijing, CN;
Zuoyan Zhu, Beijing, CN;
Hong Ma, Beijing, CN;
Bo Guan, Beijing, CN;
Yuanlei Hu, Beijing, CN;
Zhongping Lin, Beijing, CN;
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, CN;
Peking University, Beijing, CN;
Abstract
The invention discloses a recombinant gene which enhances the ability of fish to tolerate low dissolved oxygen (DO) stress and the use thereof. Carp β-actin gene promoter is used as a promoter andhemoglobin gene is used as a target gene, so as to construct the recombinanthemoglobin gene driven by carp β-actin promoter. The modeling organism zebrafish is used as the research object, and the recombinant gene is microinjected into zygotes of zebrafish. After PCR screening and 156 h low DO stress test, transgenic fish are obtained with a survival rate of 92%, which is significantly different from the survival rate of 65% of the control fish group. The vhb transgenic zebrafish obtain hypoxia tolerance. When the recombinant gene is applied to the economically farmed species, i.e., blunt snout bream () and common carp (L.), it enhances their hypoxia tolerance as well. Such genetically improved breeding technique may be widely used for breeding new excellent farmed species with the hypoxia tolerance.