The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 24, 2013
Filed:
Jan. 02, 2013
Applicant:
Emd Millipore Corporation, Billerica, MA (US);
Inventors:
Assignee:
EMD Millipore Corporation, Billerica, MA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/20 (2006.01); C12Q 1/02 (2006.01); C12Q 1/22 (2006.01);
U.S. Cl.
CPC ...
Abstract
A method for retention testing sterilizing grade filters comprises: a) providing a stock of; b) growing up the stock offor about 24 hours or less in a single serum-free growth medium that supports cell growth to a high titer and yields a cellular morphology where the cells are small, deaggregated and spherical, thereby producing a bacterial culture; c) challenging a test filter by filtering the bacterial culture through the test filter at a known challenge level, thereby producing a filtrate downstream of the test filter; and d) detecting concentration ofin the filtrate. Serum-free growth media for cultivating or storingare also described.