The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 03, 2013
Filed:
Apr. 27, 2010
Paul Pevsner, New York, NY (US);
Frederick Naftolin, Woodbridge, CT (US);
Douglas C. Miller, Belle Mead, NJ (US);
Dean Hillman, New Hyde Park, NY (US);
Brian K. Stall, Bedford, NH (US);
Steven M. Wishnies, Columbia, MD (US);
Paul Pevsner, New York, NY (US);
Frederick Naftolin, Woodbridge, CT (US);
Douglas C. Miller, Belle Mead, NJ (US);
Dean Hillman, New Hyde Park, NY (US);
Brian K. Stall, Bedford, NH (US);
Steven M. Wishnies, Columbia, MD (US);
New York University, New York, NY (US);
Abstract
The present invention relates to the use of post source decay (PSD) or collision induced dissociation (CID) direct tissue (DT) MALDI-TOF or DT-MALDI-TOF-TOF mass spectrographic identification of biological molecules in a tissue or cellular sample without the need for further protein extraction. This method provides for studying cells or tissues by direct tissue MALDI (DT-MALDI), thereby substituting in situ protein release for further protein extraction. Mass/intensity data was processed with Mascot© software interrogation of the NCBI database. These results are proof of principle that DT-MALDI, combined with bioinformatics, can directly identify proteins in cells and tissues from their mass spectra.