The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 03, 2013
Filed:
Jan. 08, 2010
Stephen D. Dertinger, Webster, NY (US);
Sian E. Cairns, Clifton Park, NY (US);
Svetlana L. Avlasevich, Webster, NY (US);
Dorothea K. Torous, Rochester, NY (US);
Stephen D. Dertinger, Webster, NY (US);
Sian E. Cairns, Clifton Park, NY (US);
Svetlana L. Avlasevich, Webster, NY (US);
Dorothea K. Torous, Rochester, NY (US);
Litron Laboratories, Ltd., Rochester, NY (US);
Abstract
The present invention relates a method for the enumeration of mammalian cell micronuclei, while distinguishing micronuclei from the chromatin of dead and dying cells. The method utilizes differential staining of chromatin from dead and dying cells, to distinguish the chromatin from micronuclei and nuclei that can be detected based upon fluorescent emission and light scatter following exposure to an excitatory light source. Counting of micronuclei events relative to the number of nuclei can be used to assess the DNA-damaging potential of a chemical agent, the DNA-damaging potential of a physical agent, the effects of an agent which can modify endogenously-induced DNA damage, and the effects of an agent which can modify exogenously-induced DNA damage. Kits for practicing the invention are also disclosed.