The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 06, 2013

Filed:

Jan. 31, 2012
Applicants:

Weiqiang Liu, Nanjing, CN;

Ping Yang, Princeton, NJ (US);

Tao Wang, Nanjing, CN;

Zhuying Wang, Monmouth Junction, NJ (US);

Wenzhu Chen, Nanjing, CN;

Fang Liang Zhang, Fanwood, NJ (US);

Inventors:

Weiqiang Liu, Nanjing, CN;

Ping Yang, Princeton, NJ (US);

Tao Wang, Nanjing, CN;

Zhuying Wang, Monmouth Junction, NJ (US);

Wenzhu Chen, Nanjing, CN;

Fang Liang Zhang, Fanwood, NJ (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/20 (2006.01); C12N 9/18 (2006.01); C07K 17/00 (2006.01); C12N 15/64 (2006.01);
U.S. Cl.
CPC ...
Abstract

Methods and compositions for cloning a donor DNA molecule into an acceptor vector at a predetermined location are described. The methods are based on homologous recombination mediated by in vitro treatment of the donor DNA and the acceptor vector with an enzyme cocktail containing an exonuclease and a single-stranded DNA binding protein.


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