The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 06, 2013
Filed:
Mar. 23, 2004
Jochen Urthaler, Maria Enzersdorf, AT;
Roman Necina, Vienna, AT;
Christine Ascher, Kundl, AT;
Helga Woehrer, Moedling, AT;
Jochen Urthaler, Maria Enzersdorf, AT;
Roman Necina, Vienna, AT;
Christine Ascher, Kundl, AT;
Helga Woehrer, Moedling, AT;
Boehringer Ingelheim RCV GmbH & Co KG, Vienna, AT;
Abstract
A scalable process and device for producing a biomolecule, in particular pharmaceutical grade plasmid DNA. The process includes the steps of alkaline lysis and a neutralization. For separating the lysate and the precipitate, the mixture is allowed to gently flow downward through a clarification reactor that is partially filled, in its lower part, with retention material like glass beads, whereby the precipitate is retained on top of and within the retention. In a preferred embodiment of the lysis step, cell suspension and alkaline lysis solution flow through a lysis reactor that is filled with particulate material like glass beads. The process can be run continuously and fully automated.