The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 23, 2013

Filed:

Jan. 02, 2008
Applicants:

Alain Lejars, Pierres, FR;

Michel Nogre, Vanves, FR;

Monique Ollivier, Le Kremlin Bicetre, FR;

Inventors:

Alain Lejars, Pierres, FR;

Michel Nogre, Vanves, FR;

Monique Ollivier, Le Kremlin Bicetre, FR;

Assignee:

LFB Biotechnologies, Les Ulis, FR;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 14/745 (2006.01); A23C 9/148 (2006.01); A23J 1/20 (2006.01);
U.S. Cl.
CPC ...
Abstract

The invention relates to a method for extracting a protein from milk, having at least one hydrophobic pocket and a negative charge to the natural pH of milk, that comprises the following steps: a) skimming and delipidation of the milk; b) passing the delipidated and skimmed fraction containing the protein on a chromatographic substrate on which is grafted a ligand having both a hydrophobic characteristic and an ionic characteristic in pH conditions enabling the protein to be retained on the substrate, the pH being higher than 4.6; c) elution of the protein; d) purification of the eluted fraction by removing the milk proteins from the eluted fraction; and e) recovering the protein.


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