The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 16, 2013
Filed:
Sep. 14, 2007
Nicolas Ugolin, Paris, FR;
Denis Menut, Creteil, FR;
Julien Le Meur, Pont-Aven, FR;
Pascal Wodling, Orsay, FR;
Sylvie Chevillard, Le Kremlin-Bicetre, FR;
Nicolas Ugolin, Paris, FR;
Denis Menut, Creteil, FR;
Julien Le Meur, Pont-Aven, FR;
Pascal Wodling, Orsay, FR;
Sylvie Chevillard, Le Kremlin-Bicetre, FR;
Commissariat a l'Energie Atomique, Paris, FR;
Abstract
The present invention relates in particular to a method for the quantitative measurement of biomolecular targets that have been deposited on a biochip () of the type with a matrix of probes hybridized by the targets, the matrix comprising a multitude of measurement points () each comprising a plurality of probes, characterized in that it comprises the following steps: a) at least one laser beam () is focused onto each measurement point, in order to extract therefrom a hot confined plasma comprising a chemical element to be quantified that is present in the targets and optionally in the probes; b) the light emission lines from the plasma are detected and analysed for each measurement point, by measuring the respective intensities of these lines; and then c) the concentration in each measurement point of the element or of a group incorporating it within the targets is determined via a prior calibration of the lines establishing a correlation between the intensities of the lines specific to the element to be quantified and given concentrations of this element.