The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 16, 2013
Filed:
Jan. 23, 2012
Jeannine C. Muller-greven, Mentor, OH (US);
Marc A. Post, Beachwood, OH (US);
Christopher J. Kubu, Twinsburg, OH (US);
Jeannine C. Muller-Greven, Mentor, OH (US);
Marc A. Post, Beachwood, OH (US);
Christopher J. Kubu, Twinsburg, OH (US);
Affymetrix, Inc., Santa Clara, CA (US);
Abstract
A heat labile alkaline phosphatase enzyme and methods of using the same and kits including the same are disclosed. Specifically, a nucleotide sequence of, peptide sequence of, methods of using, and kits comprising, a heat labile alkaline phosphatase isolated fromare provided. Methods of over-expression and purification of the recombinant alkaline phosphatase and mutants thereof are also disclosed. Methods of over-expressing and purifying commercially useful quantities of active recombinant heat labile alkaline phosphatase fusion enzymes from, wherein the fusion enzymes comprise one or more heterologous leader sequences are disclosed. The disclosedheat labile alkaline phosphatase has properties similar to shrimp alkaline phosphatase and can be substituted for shrimp alkaline phosphatase in assays involving the same.