The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 16, 2013
Filed:
Jun. 24, 2008
Aldo Paolicchi, San Giuliano Terme, IT;
Alfonso Pompella, Pisa, IT;
Maria Franzini, Calci, IT;
Renata Barsacchi, Localita S. Maria del Giudice, IT;
Michele Emdin, Pisa, IT;
Emilia Bramanti, Pietrasanta, IT;
Aldo Paolicchi, San Giuliano Terme, IT;
Alfonso Pompella, Pisa, IT;
Maria Franzini, Calci, IT;
Renata Barsacchi, Localita S. Maria del Giudice, IT;
Michele Emdin, Pisa, IT;
Emilia Bramanti, Pietrasanta, IT;
Universita' di Pisa, Pisa, IT;
Abstract
A method is described for detecting the gamma-glutamyl transferase enzyme isoforms (GGT, EC 2.3.2.2) in a sample of biological fluid, such as for example plasma or serum. The method comprises an HPLC separation step of the sample proteins based on the molecular size and a second step for detecting the GGT isoforms by post-column reaction with a GGT enzyme substrate capable of generating a detectable final product, preferably by spectrophotometric or fluorimetric means. The GGT isoforms can be separated by ultra-centrifugation, thereby obtaining three enzymatic isoforms characterized by molecular weights of approximately 2000, 940, and 140 KDa, respectively.