The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 09, 2013
Filed:
Apr. 21, 2010
Applicant:
Junichi Takagi, Osaka, JP;
Inventor:
Junichi Takagi, Osaka, JP;
Assignee:
Osaka University, Osaka, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/07 (2010.01); C07K 4/00 (2006.01); C12P 21/08 (2006.01);
U.S. Cl.
CPC ...
Abstract
Provided is a tag peptide in which a protease recognition sequence and an epitope of an antibody against the tag peptide are overlapped and thereby the protease recognition sequence per se is usable for detection or purification. Also provided is a purification method for recombinant proteins using the tag peptide and an antibody thereagainst. A preferable tag peptide comprises a Tobacco etch virus (TEV) protease recognition sequence as the protease recognition sequence, and its examples include a tag peptide comprising the amino acid sequence (1): RXXLYXQGKDG (wherein X, Xand Xmay be the same or different and represent any amino acid residue).