The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 09, 2013
Filed:
Feb. 17, 2011
Plamen A. Demirev, Ellicott City, MD (US);
Nathan A. Hagan, Ellicott City, MD (US);
Miquel D. Antoine, Columbia, MD (US);
Jeffrey S. Lin, Silver Spring, MD (US);
Andrew B. Feldman, Columbia, MD (US);
Plamen A. Demirev, Ellicott City, MD (US);
Nathan A. Hagan, Ellicott City, MD (US);
Miquel D. Antoine, Columbia, MD (US);
Jeffrey S. Lin, Silver Spring, MD (US);
Andrew B. Feldman, Columbia, MD (US);
The Johns Hopkins University, Baltimore, MD (US);
Abstract
The present invention is based on the discovery that drug resistance in microorganisms can be rapidly and accurately determined using mass spectrometry. A mass spectrum of an intact microorganism or one or more isolated biomarkers from the microorganism grown in drug containing, isotopically-labeled media is compared with a mass spectrum of the intact microorganism or one or more isolated biomarkers from the microorganism grown in non-labeled media without the drug present. Drug resistance is determined by predicting and detecting a characteristic mass shift of one or more biomarkers using algorithms. The characteristic mass shift is indicative that the microorganism is growing in the presence of the drug and incorporating the isotopic label into the one or more biomarkers, resulting in change in mass.