The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 09, 2013
Filed:
Aug. 21, 2009
Richard Gniewek, Oro Valley, AZ (US);
Michael Farrell, Tucson, AZ (US);
Hiroaki Nitta, Oro Valley, AZ (US);
Megan Lehrkamp, Castro Valley, CA (US);
Jerome Kosmeder, Tucson, AZ (US);
Brian Daniel Kelly, Tucson, AZ (US);
Thomas Grogan, Tucson, AZ (US);
Fabien Gaire, Oro Valley, AZ (US);
Mary Padilla, Tucson, AZ (US);
Christopher Bieniarz, Tucson, AZ (US);
Richard Gniewek, Oro Valley, AZ (US);
Michael Farrell, Tucson, AZ (US);
Hiroaki Nitta, Oro Valley, AZ (US);
Megan Lehrkamp, Castro Valley, CA (US);
Jerome Kosmeder, Tucson, AZ (US);
Brian Daniel Kelly, Tucson, AZ (US);
Thomas Grogan, Tucson, AZ (US);
Fabien Gaire, Oro Valley, AZ (US);
Mary Padilla, Tucson, AZ (US);
Christopher Bieniarz, Tucson, AZ (US);
Ventana Medical Systems, Inc., Tucson, AZ (US);
Abstract
The present invention provides a method and kit for detection of two or more target molecules in a single tissue sample, such as for gene and protein dual detection in a single tissue sample. Methods comprise treating a tissue sample with a first binding moiety that specifically binds a first target molecule. Methods further comprise treating the tissue sample with a solution containing a soluble electron-rich aromatic compound prior to or concomitantly with contacting the tissue sample with a hapten-labeled binding moiety and detecting a second target molecule. In one example, the first target molecule is a protein and the second is a nucleic acid sequence, the first target molecule being detected by immunohistochemistry and the second by in situ hybridization. The disclosed method reduces background due to non-specific binding of the hapten-labeled specific binding moiety to an insoluble electron rich compound deposited near the first target molecule.