The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 09, 2013
Filed:
Sep. 18, 2009
Keith Bjornson, Newark, CA (US);
Arek Bibillo, Cupertino, CA (US);
Fred Christians, Los Altos Hills, CA (US);
Kevin Travers, Santa Clara, CA (US);
Robin Emig, Belmont, CA (US);
Keith Bjornson, Newark, CA (US);
Arek Bibillo, Cupertino, CA (US);
Fred Christians, Los Altos Hills, CA (US);
Kevin Travers, Santa Clara, CA (US);
Robin Emig, Belmont, CA (US);
Pacific Biosciences of California, Inc., Menlo Park, CA (US);
Abstract
Provided are methods for sequencing a nucleic acid that include fixing a template to a surface through a template localizing moiety and sequencing the nucleic acid with a sequencing enzyme, e.g. a polymerase or exonuclease. The sequencing enzyme can optionally be exchanged with a second sequencing enzyme, which continues the sequencing of the nucleic acid. The template localizing moiety can optionally anneal with the nucleic acid and/or associate with the sequencing enzyme. Also provided are compositions comprising a nucleic acid fixed to a surface via a template localizing moiety, and a first sequencing enzyme, which can sequence the nucleic acid and optionally exchange with a second sequencing enzyme present in the composition. Compositions in which a template localizing moiety is immobilized on a surface are provided. Compositions for sequencing reactions are provided. Also provided are sequencing systems comprising reaction regions in which or near which template localizing moieties are immobilized.