The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 02, 2013

Filed:

Jun. 02, 2011
Applicants:

Chungen Qian, Shenzhen, CN;

KE LI, Shenzhen, CN;

Yuping Zhang, Shenzhen, CN;

Inventors:

Chungen Qian, Shenzhen, CN;

Ke Li, Shenzhen, CN;

Yuping Zhang, Shenzhen, CN;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/96 (2006.01); C07K 1/107 (2006.01);
U.S. Cl.
CPC ...
Abstract

Methods for preparing conjugates including enzyme conjugates and especially alkaline phosphatase (ALP) conjugates, and a kit are provided. The methods include: blocking an amino group on a molecule surface of a first substance to be conjugated containing an amino group and a carboxyl group (for example, an enzyme) with a carboxyl compound; adding a carbodiimide to activate the first substance to be conjugated with the amino group blocked; inactivating or removing the carbodiimide; and adding a second substance containing an amino group (for example, a substance to be labeled). Conjugates (for example, enzyme conjugates) are obtained.


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