The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 18, 2013
Filed:
Sep. 12, 2008
Nathalie Lenne, Muttersholtz, FR;
Patrick Erbacher, Benfeld, FR;
Nathalie Lenne, Muttersholtz, FR;
Patrick Erbacher, Benfeld, FR;
Polyplus Transfection SA, Illkirch Cedex, FR;
Centre National de la Recherche Scientifique, Paris Cedex, FR;
Universite de Strasbourg, Strasbourg, FR;
Abstract
The invention relates to a method for manipulating, isolating, detecting or amplifying a target nucleic acid in a sample by hybridization with an oligonucleotide-oligocation conjugate, comprising allowing said nucleic acid to react with an oligonucleotide-oligocation conjugate comprising at least A1 and Bj linked together directly or via a linker, wherein. A, is an i-mer oligonucleotides, with i=3 to 50, where Ai is an oligomer with naturally or non naturally occurring nucleobases and/or pentafuranosyl groups and/or native phosphodiester bonds, optionally comprising a marker group. Bj is a j-mer organic oligocation moiety, with j=1 to 50, where B is —HPO—R—(NH—R)—NH—R—O—, where R, Rand Rare lower alkylene, identical or different, NH—R2 moieties being identical or different when n is >1; HPO—R—CH(X)—R—O—, where Ri and R3, identical or different, are lower alkylene and X is putrescine, spermidine or spermine residue.