The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 21, 2013
Filed:
Aug. 16, 2010
Katharine J. Gibson, Wilmington, DE (US);
Der-ing Liao, Wilmington, DE (US);
Xiao-song Tang, Hockessin, DE (US);
Katharine J. Gibson, Wilmington, DE (US);
Der-Ing Liao, Wilmington, DE (US);
Xiao-Song Tang, Hockessin, DE (US);
E I du Pont de Nemours and Company, Wilmington, DE (US);
Abstract
Sequences of B-dependent dehydratases with improved reaction kinetics are presented. Use of these B-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the α-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.