The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 14, 2013
Filed:
Mar. 03, 2005
Vladimir L. Makarov, Ann Arbor, MI (US);
Emmanuel Kamberov, Ann Arbor, MI (US);
Tong Sun, Novi, MI (US);
Jonathon H. Pinter, Ypsilanti, MI (US);
Brendan J. Tarrier, Whitmore Lake, MI (US);
Eric E. Bruening, Chelsea, MI (US);
Takao Kurihara, Ann Arbor, MI (US);
Tim Tesmer, Whittaker, MI (US);
Joseph M'mwirichia, Ypsilanti, MI (US);
Vladimir L. Makarov, Ann Arbor, MI (US);
Emmanuel Kamberov, Ann Arbor, MI (US);
Tong Sun, Novi, MI (US);
Jonathon H. Pinter, Ypsilanti, MI (US);
Brendan J. Tarrier, Whitmore Lake, MI (US);
Eric E. Bruening, Chelsea, MI (US);
Takao Kurihara, Ann Arbor, MI (US);
Tim Tesmer, Whittaker, MI (US);
Joseph M'Mwirichia, Ypsilanti, MI (US);
Rubicon Genomics, Ann Arbor, MI (US);
Abstract
The present invention regards a variety of methods and compositions for obtaining epigenetic information, such as DNA methylation patterns, through the preparation, amplification and analysis of Methylome libraries. In particular, the method employs preparation of a DNA molecule by digesting the DNA molecule with at least one methylation-sensitive restriction enzyme; incorporating a nucleic acid molecule into at least some of the digested DNA molecules by either (1) incorporating at least one primer from a plurality of primers that have a 5' constant sequence and a 3′ variable sequence, wherein the primers are substantially non-self-complementary and substantially non-complementary to other primers in the plurality; or (2) incorporating an oligonucleotide having an inverted repeat and a loop under conditions wherein the oligonucleotide becomes blunt-end ligated to one strand of the digested DNA molecule, followed by polymerization from a 3′ hydroxyl group present in a nick in the oligonucleotide-linked molecule; and amplifying one or more of the DNA molecules.