The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 07, 2013
Filed:
Aug. 28, 2009
Seung-pyo Hong, Hockessin, DE (US);
Quinn Qun Zhu, West Chester, PA (US);
John E. Seip, Alloway, NJ (US);
Seung-Pyo Hong, Hockessin, DE (US);
Quinn Qun Zhu, West Chester, PA (US);
John E. Seip, Alloway, NJ (US);
E I du Pont de Nemours and Company, Wilmington, DE (US);
Abstract
Methods of increasing the total lipid content in a eukaryotic cell, the total content of polyunsaturated fatty acids ['PUFAs'], and/or the ratio of desaturated fatty acids to saturated fatty acids by reducing the activity of the heterotrimeric SNF1 protein kinase are disclosed. Preferably, the chromosomal genes encoding the Snf1 α-subunit, Gal83 β-subunit or Snf4 γ-subunit of the SNF1 protein kinase, the upstream regulatory genes encoding Sak1, Hxk2, Glk1 or Reg1, or the downstream genes encoding Rme1, Cbr1 or Snf3 are manipulated in a PUFA-producing strain of the oleaginous yeast, resulting in increased total lipid content, as compared to the parent strain comprising the heterotrimeric SNF1 protein kinase not having reduced activity.