The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 09, 2013

Filed:

Aug. 31, 2004
Applicants:

Hidekazu Hiroaki, Yokohama, JP;

Takeshi Tenno, Yokohama, JP;

Natsuko Goda, Yokohama, JP;

Inventors:

Hidekazu Hiroaki, Yokohama, JP;

Takeshi Tenno, Yokohama, JP;

Natsuko Goda, Yokohama, JP;

Assignee:

Yokohama City University, Kanagawa, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/00 (2006.01); C12N 15/70 (2006.01); C12N 15/72 (2006.01);
U.S. Cl.
CPC ...
Abstract

The present invention provides a tool for reducing the time and labor required for obtaining a protein by recombinant expression of a gene in a host. A vector characterized by having two restriction recognition sequences different in nucleotide sequence both of which are recognized by a first restriction enzyme, one of the restriction recognition sequences comprising a part of a restriction recognition sequence recognized by a second restriction enzyme that is different from the first restriction enzyme, and the other one of the restriction recognition sequences not comprising the part of the restriction recognition sequence recognized by the second restriction enzyme. A method of producing a protein and/or a protein domain, using the above-described vector.


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