The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 19, 2013
Filed:
Mar. 07, 2008
Carl T. Wittwer, Salt Lake City, UT (US);
Luming Zhou, Salt Lake City, UT (US);
Mark Aaron Poritz, Salt Lake City, UT (US);
Carl T. Wittwer, Salt Lake City, UT (US);
Luming Zhou, Salt Lake City, UT (US);
Mark Aaron Poritz, Salt Lake City, UT (US);
University of Utah Research Foundation, Salt Lake City, UT (US);
BioFire Diagnostics, Inc., Salt Lake City, UT (US);
Abstract
Methods and kits are provided for nucleic acid analysis. In an illustrative method a target nucleic acid is amplified using a first primer and a second primer, wherein the first primer comprises a probe element specific for a locus of the target nucleic acid and a template-specific primer region, and the probe element is 5' of the template-specific primer region, subsequently allowing the probe element to hybridize to the locus to form a hairpin, generating a melting curve for the probe element by measuring fluorescence from a dsDNA binding dye as the mixture is heated, wherein the dye is not covalently bound to the first primer, and analyzing the shape of the melting curve. Kits may include one or more of the first and second primers, the dsDNA binding dye, a polymerase, and dNTPs.