The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 12, 2013

Filed:

Mar. 10, 2010
Applicants:

Thomas Tuschl, Brooklyn, NY (US);

John Pena, New York, NY (US);

Cherin Sohn, Rego Park, NY (US);

Sara Hakim, New York, NY (US);

Janos Ludwig, Bonn-Venusberg, DE;

Pavol Cekan, New York, NY (US);

Inventors:

Thomas Tuschl, Brooklyn, NY (US);

John Pena, New York, NY (US);

Cherin Sohn, Rego Park, NY (US);

Sara Hakim, New York, NY (US);

Janos Ludwig, Bonn-Venusberg, DE;

Pavol Cekan, New York, NY (US);

Assignee:

The Rockefeller University, New York, NY (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); G01N 33/48 (2006.01);
U.S. Cl.
CPC ...
Abstract

In one aspect, the invention relates to a method for fixing a short nucleic acid in a biological sample. In another aspect, the invention relates to a method for detecting a target short nucleic acid in a biological sample. The method includes contacting the biological sample with an aldehyde-containing fixative, and subsequently contacting the sample with a water-soluble carbodiimide. In a further aspect, the invention relates to a kit for fixing a short nucleic acid in a biological sample. The kit includes a support substrate for holding the sample; an aldehyde-containing fixative; and a water-soluble carbodiimide.


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