The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 12, 2013

Filed:

Dec. 18, 2009
Applicants:

Gregory Timp, South Bend, IN (US);

Winston Timp, Baltimore, MD (US);

Andrew Feinberg, Baltimore, MD (US);

Utkur Mirsaidov, Urbana, IL (US);

Inventors:

Gregory Timp, South Bend, IN (US);

Winston Timp, Baltimore, MD (US);

Andrew Feinberg, Baltimore, MD (US);

Utkur Mirsaidov, Urbana, IL (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C12P 19/34 (2006.01); C12M 1/34 (2006.01); C07H 21/02 (2006.01); C07H 19/00 (2006.01);
U.S. Cl.
CPC ...
Abstract

Provided are methods for detecting, characterizing or separating DNA based on methylation. Heterogeneous DNA populations are separated based on DNA methylation by providing a membrane having a nanopore through which an electric field is applied. DNA of interest is introduced, and for a given threshold voltage across the nanopore, only DNA having a methylation parameter of interest may transit the pore, thereby facilitating detection, characterization, or separation of DNA based on methylation. The methods are optionally used to detect a disease state that is associated with DNA methylation including, but not limited to, cancer.


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