The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 26, 2013
Filed:
Aug. 22, 2008
Miroslaw Bartkowiak, Raleigh, NC (US);
Richard L. Moore, Glenville, PA (US);
Miroslaw Bartkowiak, Raleigh, NC (US);
Richard L. Moore, Glenville, PA (US);
Becton, Dickinson and Company, Franklin Lakes, NJ (US);
Abstract
Systems and methods for calculating an initial amount of target nucleic acid Nin a sample are provided. A plurality of fluorescent measurements is received. Each respective fluorescent measurement FSis taken in a different cycle n in a PCR amplification experiment of the sample. Then, a model for the PCR amplification experiment is computed. For each respective fluorescent measurement, the model comprises a respective equation for N, where (i) Nis the calculated amount of the target nucleic acid in cycle n of the corresponding PCR amplification experiment, and (ii) the equation for Nis expressed in terms of K and N, where K is the Michaelis-Menton constant. The model can be refined by adjusting K and Nuntil differences between model values Nand corresponding fluorescent measurements are minimized, thereby calculating the initial amount of a target nucleic acid Nas the minimized value for Nfor the model.