The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 26, 2013
Filed:
Dec. 22, 2009
Mary Frances Lopez, Boston, MA (US);
David A. Sarracino, Andover, MA (US);
Amol Prakash, Cambridge, MA (US);
Randall W. Nelson, Phoenix, AZ (US);
Paul Oran, Scottsdale, AZ (US);
Bryan E. Krastins, Somerville, MA (US);
Taha Rezai, Cambridge, MA (US);
Mary Frances Lopez, Boston, MA (US);
David A. Sarracino, Andover, MA (US);
Amol Prakash, Cambridge, MA (US);
Randall W. Nelson, Phoenix, AZ (US);
Paul Oran, Scottsdale, AZ (US);
Bryan E. Krastins, Somerville, MA (US);
Taha Rezai, Cambridge, MA (US);
Thermo Finnigan, LLC, San Jose, CA (US);
Intrinsic Bioprobes, Inc., Tempe, AZ (US);
Abstract
Methods are described for monitoring the amounts of PTH variants in a biological sample by digesting the sample to produce surrogate peptides specific to the targeted PTH variants, and detecting and quantifying the surrogate peptides by selective reaction monitoring (SRM) mass spectrometry, using a set of precursor-to-product ion transitions optimized for sensitivity and selectivity. The PTH variants, or a portion thereof, may be concentrated in the sample by means of immunoaffinity capture or other suitable technique. The mass spectrometric method described herein enables the concurrent measurement of peptides representative of a plurality of targeted PTH variants in a single assay.