The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 19, 2013
Filed:
Dec. 23, 2008
Young-doug Sohn, Yongin-si, KR;
Bum Joon Kim, Seoul, KR;
OK Hwan Kim, Seoul, KR;
Kyoung Jun Kim, Seoul, KR;
Ji Hun Shin, Seoul, KR;
Sung Yu Hong, Seoul, KR;
Jae Hoon Hwang, Seoul, KR;
Kwang Hoe Chung, Seongnam-si, KR;
Young-Doug Sohn, Yongin-si, KR;
Bum Joon Kim, Seoul, KR;
Ok Hwan Kim, Seoul, KR;
Kyoung Jun Kim, Seoul, KR;
Ji Hun Shin, Seoul, KR;
Sung Yu Hong, Seoul, KR;
Jae Hoon Hwang, Seoul, KR;
Kwang Hoe Chung, Seongnam-si, KR;
Biobud Co., Ltd, Seoul, KR;
Abstract
The present invention relates to a batroxobin-encoding nucleotide sequence and/or a mutated α-factor secretion signal sequence, and a vector and a transformant using the same. The batroxobin-encoding nucleotide sequence of this invention exhibits an excellent expression efficiency in yeast, particularand the recombinant batroxobin is obtained at 4-13 fold higher yield than natural-occurring batroxobin-encoding sequences. The protein expression system which uses the batroxobin-encoding nucleotide sequence as well as mutated α-factor secretion signal peptide sequence of this invention obtains the recombinant batroxobin at about 20-fold higher yield than natural-occurring batroxobin-encoding sequences. In addition, the recombinant batroxobin prepared using the sequence of this invention has a significantly plausible activity and stability compared with natural-occurring batroxobin.