The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 12, 2013
Filed:
Apr. 30, 2012
Scott J. Geromanos, Middletown, NJ (US);
Marc Victor Gorenstein, Needham, MA (US);
Jeffrey Cruz Silva, Beverly, MA (US);
Guo-zhong LI, Westborough, MA (US);
Scott J. Geromanos, Middletown, NJ (US);
Marc Victor Gorenstein, Needham, MA (US);
Jeffrey Cruz Silva, Beverly, MA (US);
Guo-Zhong Li, Westborough, MA (US);
Waters Technologies Corporation, Milford, MA (US);
Abstract
Protein identification in a complex sample begins by selecting a database having proteins likely to be in the sample. In-silico digestion is performed and a target peptide is selected from produced peptides. The masses of the Y- and B-ion fragments of the target peptide are determined. These masses are used to search previously obtained low- and high-energy AMRTs obtained from LC/MS analysis of the complex sample for masses on the list. Any mass observed in the data within a detection threshold are considered a hit. If enough hits accumulate in a given retention time, the target peptide is identified as being in the sample. The list of peptides identified in the complex sample can be used to identify the proteins present in the sample, track the chromatographic retention times of peptides between samples, and quantitate the peptides and proteins present in complex samples.