The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 12, 2013
Filed:
Jan. 11, 2012
Alois Jungbauer, Vienna, AT;
Rainer Hahn, Vienna, AT;
Anne Tscheliessnig, Ledenitzen, AT;
Waltraud Kaar, Indooroopilly, AU;
Alois Jungbauer, Vienna, AT;
Rainer Hahn, Vienna, AT;
Anne Tscheliessnig, Ledenitzen, AT;
Waltraud Kaar, Indooroopilly, AU;
Sandoz AG, Basel, CH;
Boehringer Ingelheim RCV GmbH & Co KG, Vienna, AT;
Abstract
Disclosed is a method for the production of a heterologous polypeptide of interest with a homogenous N-terminus, using a fusion polypeptide comprising the polypeptide of interest and N-terminally thereto a polypeptide exhibiting autoproteolytic function, said method comprises the steps of a) binding of the fusion polypeptide in a soluble, autoproteolytically inactive form by an affinity chromatography system, b) refolding of the fusion polypeptide, thereby activating the autoproteolytic function of the fusion polypeptide and causing cleavage of the heterologous polypeptide of interest, and c) subsequently eluting the heterologous polypeptide of interest, wherein the steps are conducted on one affinity chromatography system.