The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 05, 2013
Filed:
Jul. 17, 2002
Francis Barany, New York, NY (US);
Jianzhao Kiu, Boston, MA (US);
Brian W. Kirk, New York, NY (US);
Monib Zirvi, Willingboro, NJ (US);
Norman P. Gerry, Boston, MA (US);
Philip B. Paty, New York, NY (US);
Francis Barany, New York, NY (US);
Jianzhao Kiu, Boston, MA (US);
Brian W. Kirk, New York, NY (US);
Monib Zirvi, Willingboro, NJ (US);
Norman P. Gerry, Boston, MA (US);
Philip B. Paty, New York, NY (US);
Cornell Research Foundation, Inc., Ithaca, NY (US);
Sloan-Kettering Institute for Cancer Research, New York, NY (US);
Abstract
The present invention is directed to a method of assembling genomic maps of an organism's DNA or portions thereof. A library of an organism's DNA is provided where the individual genomic segments or sequences are found on more than one clone in the library. Representations of the genome are created, and nucleic acid sequence information is generated from the representations. The sequence information is analyzed to determine clone overlap from a representation. The clone overlap and sequence information from different representations is combined to assemble a genomic map of the organism. Once the genomic map is obtained, genomic sequence information from multiple individuals can be applied to the map and compared with one another to identify single nucleotide polymorphisms. These single nucleotide polymorphisms can be detected, and alleles quantified, by conducting (1) a global PCR amplification which creates a genome representation, and (2) a ligation detection reaction process whose ligation products are captured by hybridization to a support.